EHC-93, EHC-98, EHC-2000, SRM-1648, SRM-1649 or TiO2 (?), Tukey test, p?p?Gja7 represents the higher estimate (IL-10 potency subtracted, IL-6 added to average potency) (c) Similarly, an inflammatory potency estimate I-V CELLS, was obtained for each particle by averaging the cell viability-adjusted inflammatory potency values Ziprasidone D8 of the particles across both cell lines for all cytokines detected, for each inflammatory scenario (Fig.?5b). The estimates impacted the magnitude of particle potency ranking, but the ranking was comparable between the different scenarios, where the urban particles, except EHC-93 were more potent than mineral particles and DWR1. Lastly, an overall integrated potency estimate, I CELLS of the particles was calculated by averaging the biological reactivity, R CELLS with inflammatory indices, I-V CELLSfor each particle (Fig.?5c). The I CELLS profile was similar to the profile of the inflammatory indices I-V CELLS. Toxicity of particles in vivo BALB/c mice were exposed for 24?h to a subset of particles selected based on contrasting in vitro potency (EHC-2000, SRM-1649, CRI, TiO2 and DWR1) by intratracheal instillation (IT). Ziprasidone D8 Acute pulmonary toxicity was observed in mice exposed to the high dose (250?g) of the particles, as shown by a general increase.